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primary human preadipocytes  (PromoCell)


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    Structured Review

    PromoCell primary human preadipocytes
    Primary Human Preadipocytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+preadipocytes/HWP-c+Human+White+Preadipocytes%2C+subcutaneous%2C+cryopreserved/pm41171875-96-0-3
    Average 93 stars, based on 14 article reviews
    primary human preadipocytes - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: ATGL activity regulates GLUT1-mediated glucose uptake and lactate production via TXNIP stability in adipocytes
    Article Snippet: .. Primary human preadipocytes were purchased from Promo cell (C-12730) and cultured according to manufacturer's instructions. ..

    Article Title: ATGL activity regulates GLUT1-mediated glucose uptake and lactate production via TXNIP stability in adipocytes
    Article Snippet: .. Primary human preadipocytes were purchased from Promo cell (C-12730) and cultured according to manufacturer’s instructions. ..

    Article Title: PKA regulatory subunit R2B is required for murine and human adipocyte differentiation
    Article Snippet: The samples were digested with collagenase type 2 (Sigma–Aldrich), and preadipocytes were isolated as described previously (12), on the basis of cell dimension and density. .. Primary human preadipocytes were cultured until they reached the total confluence stage in complete Preadipocyte Growth Medium (PromoCell GmbH, Heidelberg, Germany), a low-serum medium optimized for primary human cells containing DMEM F12 with 5% fetal calf serum (FCS), 0.4% endothelial cell growth supplement, 10 ng/ml epidermal growth factor (recombinant human), and 1 mg/ml hydrocortisone. .. After confluence, the growth medium was replaced with Preadipocyte Differentiation Medium (Promocell GmbH), a serum-free medium containing DMEM F12 with 8 mg/ml D-biotin, 0.5 mg/ml insulin (recombinant human), 400 ng/ml dexamethasone, 44 mg/ml IBMX, 9 ng/ml L-thyroxine, and 3 mg/ml ciglitazone.

    Article Title: PKA regulatory subunit R2B is required for murine and human adipocyte differentiation
    Article Snippet: The samples were digested with collagenase type 2 (Sigma–Aldrich), and preadipocytes were isolated as described previously , on the basis of cell dimension and density. .. Primary human preadipocytes were cultured until they reached the total confluence stage in complete Preadipocyte Growth Medium (PromoCell GmbH, Heidelberg, Germany), a low-serum medium optimized for primary human cells containing DMEM F12 with 5% fetal calf serum (FCS), 0.4% endothelial cell growth supplement, 10 ng/ml epidermal growth factor (recombinant human), and 1 μg/ml hydrocortisone. .. After confluence, the growth medium was replaced with Preadipocyte Differentiation Medium (Promocell GmbH), a serum-free medium containing DMEM F12 with 8 μg/ml d -biotin, 0.5 μg/ml insulin (recombinant human), 400 ng/ml dexamethasone, 44 μg/ml IBMX, 9 ng/ml l -thyroxine, and 3 μg/ml ciglitazone.

    Recombinant:

    Article Title: PKA regulatory subunit R2B is required for murine and human adipocyte differentiation
    Article Snippet: The samples were digested with collagenase type 2 (Sigma–Aldrich), and preadipocytes were isolated as described previously (12), on the basis of cell dimension and density. .. Primary human preadipocytes were cultured until they reached the total confluence stage in complete Preadipocyte Growth Medium (PromoCell GmbH, Heidelberg, Germany), a low-serum medium optimized for primary human cells containing DMEM F12 with 5% fetal calf serum (FCS), 0.4% endothelial cell growth supplement, 10 ng/ml epidermal growth factor (recombinant human), and 1 mg/ml hydrocortisone. .. After confluence, the growth medium was replaced with Preadipocyte Differentiation Medium (Promocell GmbH), a serum-free medium containing DMEM F12 with 8 mg/ml D-biotin, 0.5 mg/ml insulin (recombinant human), 400 ng/ml dexamethasone, 44 mg/ml IBMX, 9 ng/ml L-thyroxine, and 3 mg/ml ciglitazone.

    Article Title: PKA regulatory subunit R2B is required for murine and human adipocyte differentiation
    Article Snippet: The samples were digested with collagenase type 2 (Sigma–Aldrich), and preadipocytes were isolated as described previously , on the basis of cell dimension and density. .. Primary human preadipocytes were cultured until they reached the total confluence stage in complete Preadipocyte Growth Medium (PromoCell GmbH, Heidelberg, Germany), a low-serum medium optimized for primary human cells containing DMEM F12 with 5% fetal calf serum (FCS), 0.4% endothelial cell growth supplement, 10 ng/ml epidermal growth factor (recombinant human), and 1 μg/ml hydrocortisone. .. After confluence, the growth medium was replaced with Preadipocyte Differentiation Medium (Promocell GmbH), a serum-free medium containing DMEM F12 with 8 μg/ml d -biotin, 0.5 μg/ml insulin (recombinant human), 400 ng/ml dexamethasone, 44 μg/ml IBMX, 9 ng/ml l -thyroxine, and 3 μg/ml ciglitazone.

    Concentration Assay:

    Article Title: One SQ HEDGES DNA vector only dose produces durable hGLA or anti-SARS-CoV-2 mAb therapeutic serum protein levels.
    Article Snippet: .. Primary human preadipocytes (PromoCell, C-12735) were plated for differentiation at a concentration of 0.75 x105 cells per well in a 24-well plate. ..

    Article Title: One SQ HEDGES DNA vector only dose produces durable hGLA or anti-SARS-CoV-2 mAb therapeutic serum protein levels
    Article Snippet: .. Primary human preadipocytes (PromoCell, C-12735) were plated for differentiation at a concentration of 0.75 x10 5 cells per well in a 24-well plate. ..



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